Download A Practical Handbook of Preparative HPLC by Donald A Wellings PDF
By Donald A Wellings
A distillation of over 20 years of the author's sensible adventure of this procedure, this ebook intentionally steers away from advanced theoretical facets and concentrates extra at the 'black artwork' parts of the suggestions concerned. accordingly is a e-book for widespread use within the laboratory, in particular of curiosity to these within the pharmaceutical, agrochemical and biotechnology industries and to someone focused on the research and purification of substances, small molecules, proteins and DNA.
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Extra info for A Practical Handbook of Preparative HPLC
V2 V1 The measurements a, b, c and d on the chromatogram can be used to calculate peak asymmetry, A and resolution, R. Aϭ Rϭ b a 2(V2 Ϫ V1 ) (c ϩ d) Another term commonly employed is the height equivalent to a theoretical plate, HETP, which is simply the length of the column divided by the number of theoretical plates, N. The path of a molecule dissolved in the solvent passing through a packed bed is fraught with obstacles. This individual entity will have to traverse the tortuous path around the stationary phase, where the number of potential routes is numerous and at some point during that journey it may have to seek out the most inaccessible site at the centre of that particle.
2 Separation of enantiomers Enantiomers are traditionally separated by crystallization of diastereoisomeric salts or by diastereoisomeric derivatization followed by crystallization. This technique is somewhat limited if there is no acidic or basic nature or no point of derivatization. In addition, it can be a laborious process to optimize the crystallization process. 4]. 3 these has led to domination of the preparative market by two or three companies over the last 20 years. The interaction between the analyte and the stationary phase in chiral chromatography can involve characteristics of molecular size, hydrophilic, hydrophobic and ion-exchange mechanisms, often simultaneously.
13]. When describing the basic concept of the chiral stationary phase the word ‘covered’ was used deliberately since in this case, until recently, the ligand was not covalently bound to the surface of the stationary phase. The second example of process development described in Chapter 5 involves a chiral separation on this type of chiral stationary phase. 14]. 4 Ion Exchange Chromatography The majority of applications utilizing ion exchange as a mode of chromatography are generally carried out at low to medium pressure.